Phenolic trend and hygienic quality of green table olives...

来自 : www.taodocs.com/p-8966860...ht 发布时间：2021-03-25

Castro, Monta o, Casado, S nchez, Rejano, 2002; Randazzo
lation coefficient between 0.9997 and 0.9999. MRM measurements
et al., 2011). When the Optical Density measured spectrophoto-
were performed by monitoring, for each analyte, the transition of
metrically at 600 nm reached the value of 1.0, cells were harvested,
the deprotonated molecular ion [M H] 1 on the first quadrupole
washed and re-suspended in 0.9% (w/v) NaCl to obtain a final con-
and scanning its main fragments on the third quadrupole. In partic-
centration of 108 CFU mL 1 of brine. Samples not inoculated were
ular, the analytes found in brines and monitored during the six
used as controls. Three replications for both inoculated and control
months of the trial were as follows: tyrosol, hydroxytyrosol, caffeic
were performed for each cultivar.
acid, ferulic acid, apigenin, luteolin, apigenin-7-O-glucoside, luteo-
lin-4-O-glucoside, luteolin-7-O-glucoside, oleuropein and verbas-
2.3. Total soluble solids and pH of brine coside. The standards were purchased from Extrasynthese (Nord
B.P 62 69726 Genay Cedex, France) and Sigma–Aldrich (Riedel-de
The pH values of fermented brine were carried out by a pH Ha n, Laborchemikalien, Seelze). Methanol and formic acid were
meter (Crison Basic 20). Total soluble solid content was deter- LC/MS grade and purchased from VWR International; aqueous
mined using a refractometer (Atago, RX-5000, Italy) that was cali- solutions were prepared using ultrapure water, with a resistivity
brated with deionized water before samples were subjected to of 18.2 MO cm, obtained from a Milli-Q plus system (Millipore,
analysis. Bedford, MA, USA).
2.4. Total polyphenols of olives 2.6. Microbiological analyses
Total polyphenols were extracted from olive flesh following the The numbering of different microbial populations in brine was
method reported by Amiot, Fleuriette, and Macheix (1986) and carried out during fermentation. The viable mesophilic counts
measured spectrophotometrically at 725 nm after reaction with were estimated on Plate Count Agar Standard (Oxoid) incubated
the Folin–Ciocalteu’s reagent, and expressed as mg kg 1 of gallic at 32 C for 48 h. The lactic acid bacteria were grown on de Man
Table 1
Carpological characteristic of olive fruits.
Cultivar Purpose Length (mm) Diameter (mm) Fruit weight (g) Stone weight (g) Flesh weight (g) Flesh/pit
Nocellara del Belice Table 25.31 1.67 22.69 1.26 5.96 1.58 0.98 0.38 5.05 1.01 5.15 0.92
Nocellara Messinese Dual 24.93 2.19 20.02 1.72 5.40 1.33 0.90 0.22 4.50 1.20 5.00 1.02
Carolea Dual 23.76 2.06 17.38 1.44 4.48 0.97 0.72 0.16 3.76 0.85 5.22 0.83
Cassanese Oil 23.12 1.40 15.57 0.86 3.76 1.22 0.66 0.18 3.10 1.10 4.69 1.12
Data are expressed as mean standard deviation.
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